HinfI from New England Biolabs

Product Specs
ItemHinfI
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberR0155S
Concentration10-50 u/µL
Quantity5000 units
ApplicationsDNA Manipulation
Brand/TrademarkNew England Biolabs Inc (NEB)
TypeRestriction Enzyme
Temperature-20 C

Sequence

G/ANTC

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) TAR Syndrome-associated Rbm8a deficiency causes hematopoietic defects and attenuates Wnt/PCP signalingbioRxivMay 5, 2023Agnese Kocere, Elena Chiavacci, Kevin Manuel Méndez-Acevedo, Charlotte Soneson, Max S. Hiltabidle, Azhwar Raghunath, Jacalyn S. MacGowan, Jordan A. Shavit, Daniela Panáková, Margot L. K. Williams, et al.genotyped by restriction digest of the PCR product with Xmn1 (R0194S, NEB) and the rbm8aΔ3 allele by Hinf1 (R0155S, NEB) while the rbm8aΔ5 allele stayed intact upon Xmn1 or Hinf1 digest. Further, the established vangl2vu67

(2) An ultra-sensitive method to detect mutations in human RAS templatesSmall GTPasesDecember 31, 2022Siqi Li, Christopher M. Counterdilutions in Table 1). MDS assay was performed as described above using StuI (NEB R0187) or HinFI (NEB R0155) as the restriction enzyme, 60°C as exon-specific barcode primer annealing temperature, and 61°C as

(3) METTL3-mediated m6A modification stabilizes TERRA and maintains telomere stabilityNucleic acids researchNovember 11, 2022Chen L, Zhang C, Ma W, Huang J, Zhao Y, Liu H.phenol–chloroform. A 3 μg aliquot of DNAs was digested with RsaI (R0167L, New England BioLabs), HinfI (R0155L, New England BioLabs) and RNase A, and separated on a 0.7% agarose gel. After being dried and denatured,

(4) A missense variant in the nuclear localization signal of DKC1 causes Hoyeraal-Hreidarsson syndromenpj Genomic MedicineOctober 30, 2022Chia-Mei Chu, Hsin-Hui Yu, Tsai-Ling Kao, Yi-Hsuan Chen, Hsuan-Hsuan Lu, En-Ting Wu, Yun-Li Yang, Chin-Hsien Lin, Shin-Yu Lin, Meng-Ju Melody Tsai, et al.analysis Genomic DNA (2.2 µg) from iPSCs was digested with Hinf I (New England Biolabs, Cat. No: #R0155S) and Rsa I (New England Biolabs, Cat. No: #R0167L) restriction enzyme in 10X CutSmart® Buffer (NEB,

(5) The double-stranded DNA-binding proteins TEBP-1 and TEBP-2 form a telomeric complex with POT-1Nature CommunicationsDecember 1, 2021Sabrina Dietz et al.of C. elegans genomic DNA were digested in 80 µl total volume with 40 U HinfI (New England BioLabs, #R0155) and RsaI (New England BioLabs, #R0167), respectively. The digestion was incubated at 37 °C overnight

(6) Association of leukocyte telomere length with metabolic syndrome in type 2 diabetes mellitusJournal of Research in Medical SciencesJuly 31, 2021Xuemin Dong, Jiaojiao Huang, Jiaojiao Huang, Sanshan Yang, Yan YangNanodrop spectrophotometer (NanoDrop2000, Thermo Scientific, USA). Genomic DNA was digested by Hinf I (R0155 L, New England Bio Labs, Beverly, MA, USA) and RsaI (R0167 L, New England BioLabs). Agarose gel electrophoresis

(7) Telomere erosion in human pluripotent stem cells leads to ATR-mediated mitotic catastropheJournal of Cell BiologyJune 7, 2021Alexandre T. Vessoni et al.extracted DNA was digested overnight with 30 U each RsaI (#R0167; New England Biolabs) and HinfI (#R0155; New England Biolabs) restriction enzymes and resolved on a 0.8% agarose gel for 16 h at 85 V in Tris/borate/EDTA

(8) Bi-allelic MCM10 variants associated with immune dysfunction and cardiomyopathy cause telomere shorteningNature communicationsMarch 12, 2021Baxley RM, Leung W, Schmit MM, Matson JP, Yin L, Oram MK, Wang L, Taylor J, Hedberg J, Rogers CB, et al.and digested as described above. Plugs were next digested with either RsaI (NEB R0167) or HinfI (NEB R0155) restriction enzyme in 1x NEB CutSmart Buffer to degrade non-telomeric DNA. Samples were then melted,

(9) Novel and Efficient Protocol for DNA Coating- Based Identification of DNA-Protein Interaction by Antibody-Mediated ImmunodetectionReports of Biochemistry and Molecular BiologyMarch 2, 2021Niranjan Chellathurai Vasantha, Johnson Retnaraj Samuel Selvan, Saravanakumar Venkatachalam, Jackson Durairaj Selvan Christyraj, Regeneration and Stem Cell Biology Lab, Centre for Molecular and Nanomedical Sciences, International Research Centre, Sathyabama Institute of Science and Technology, Chennai, Tamilnadu, India., Regeneration and Stem Cell Biology Lab, Centre for Molecular and Nanomedical Sciences, International Research Centre, Sathyabama Institute of Science and Technology, Chennai, Tamilnadu, India., Regeneration and Stem Cell Biology Lab, Centre for Molecular and Nanomedical Sciences, International Research Centre, Sathyabama Institute of Science and Technology, Chennai, Tamilnadu, India., Regeneration and Stem Cell Biology Lab, Centre for Molecular and Nanomedical Sciences, International Research Centre, Sathyabama Institute of Science and Technology, Chennai, Tamilnadu, India.(Thermo Scientific - Cat. No. 17250). Restriction enzymes (Rsa I [NEB - Cat. No. R0167S] and Hinf I [NEB - Cat. No. R0155S]). Primary Antibodies [anti-lamin A antibody (Sigma- Cat. No. L1293) and anti-trf2 antibody (Abcam –

(10) Single cell imaging of human cancer xenografts using adult immune-deficient zebrafishNature ProtocolsSeptember 1, 2020Chuan Yan, Daniel Do, Qiqi Yang, Dalton C. Brunson, John F. Rawls, David M. Langenau(NEB, cat. no. M0273S) Nuclease-free H2O (Life Technologies, cat. no. AM9939) HinfI (NEB, cat. no. R0155S) RsaI (NEB, cat. no. R0167S) Cutsmart buffer (NEB, cat. no. B7204S) Ultrapure Agarose (Life Technologies,

(11) Shorter leucocyte telomere length as a potential biomarker for nonalcoholic fatty liver disease-related advanced fibrosis in T2DM patientsAnnals of Translational MedicineMay 2, 2020Kun Dong, Ye Zhang, Jiao-Jiao Huang, San-Shan Xia, Yan Yangextraction. The concentration of DNA samples was measured by Nanodrop. Genomic DNA were digested by Hinf I (R0155L, New England Bio Labs, Beverly, MA, USA) and RsaI (R0167L, New England BioLabs) at 37 °C overnight.

(12) FANCM suppresses DNA replication stress at ALT telomeres by disrupting TERRA R-loopsScientific reportsDecember 13, 2019Pan X, Chen Y, Biju B, Ahmed N, Kong J, Goldenberg M, Huang J, Mohan N, Klosek S, Parsa K, et al.extracted using QIAamp DNA Blood Mini Kit (Qiagen, 51106). 2 μg genomic DNA were digested with Hinf I (NEB, R0155) and Rsa I (NEB, R0167) at 37 °C overnight and then run on a 0.8% agarose gel in 1X TAE. After DNA was

(13) MTHFR C677T genetic polymorphism in combination with serum vitamin B2, B12 and aberrant DNA methylation of P16 and P53 genes in esophageal squamous cell carcinoma and esophageal precancerous lesions: a case–control studyCancer Cell InternationalDecember 1, 2019Da Pan, Ming Su, Guiling Huang, Pengfei Luo, Ting Zhang, Lingmeng Fu, Jie Wei, Shaokang Wang, Guiju Sun°C for 10 min. PCR products were digested overnight at 37 °C with restriction endonuclease Hinf I (R0155 V, New England Biolabs, UK) for recognizing and cutting the variant type sequence. The digestive PCR

(14) The Oxymonad Genome Displays Canonical Eukaryotic Complexity in the Absence of a MitochondrionMolecular Biology and EvolutionOctober 1, 2019Anna Karnkowska et al.5 µg of gDNA was digested overnight in total volume 200 µl containing five units of the HinfI (NEB, R0155S) and five units of RsaI (NEB, R0167S) restriction enzymes. DNA was purified by ethanol precipitation

(15) Association between antidiabetic agents use and leukocyte telomere shortening rates in patients with type 2 diabetesAging (Albany NY)January 28, 2019Juanhong Liu, Yuanlong Ge, Shu Wu, Delin Ma, Weijie Xu, Ye Zhang, Yan Yangprocedures using an AxyPrep Blood Genomic DNA Miniprep kit (Axygen, Corning, Inc., NY, USA). Hinf I (R0155L, New England Bio Labs, Beverly, MA, USA) and RsaI (R0167L, New England BioLabs) were used to digest

(16) Efficient mouse genome engineering by CRISPR-EZ (CRISPR RNP Electroporation of Zygotes) technologyNature ProtocolsJune 1, 2018Andrew J. Modzelewski, Sean Chen, Brandon J. Willis, K C Kent Lloyd, Joshua A. Wood, Lin Hecat. no. BP1700–100, molecular biology grade) GoTaq (Promega, cat. no. M712) HinfI (NEB, cat. no. R0155S, 10,000 units/ml) EcoRI (NEB, cat. no. R3101S, 20,000 units/ml) Additional restriction enzymes for

(17) Telomere Restriction Fragment (TRF) AnalysisBio ProtocNovember 20, 2015Ilgen Mender, Jerry W. Shay(New England BioLabs, catalog number: R0139L) HinF1 10,000 units/ml (New England BioLabs, catalog number: R0155L) MspI 20,000 units/ml (New England BioLabs, catalog number: R0106S) HaeIII 10,000 units/ml (New England BioLabs

(18) Matrix Metalloproteinases 2 and 9 Polymorphism in Patients With Myeloproliferative Diseases: A STROBE-Compliant Observational StudyMedicine (Baltimore)April 24, 2015Senem Maral, Muradiye Acar, Ozlem Sahin Balcik, Eyyup Uctepe, Omer Faruk Hatipoglu, Derya Akdeniz, Hatice Uludag Altun, Ali Kosar, Mehmet Gunduz, Esra GunduzPolymorphism Amplified genome segment was 391 bp lengths and was cut into 338 and 53 bp pieces by Hinf I (NEB R0155S) enzyme. Enzyme cutting point was T nucleotide. Length of PCR product after RFLP was 391 bp for CC genotype,

(19) LINE-1 Methylation in Plasma DNA as a Biomarker of Activity of DNA Methylation Inhibitors in Patients with Solid TumorsApril 6, 2009Ana Aparicio, Brittany North, Lindsey Barske, Xuemei Wang, Valentina Bollati, Daniel Weisenberger, Christine Yoo, Nizar Tannir, Erin Horne, Susan Groshen, et al.dehydrated to ~20μl, and then incubated with 2 μl of the restriction enzyme Hinf I (New England BioLabs, cat#R0155S) for 2 hours at 37°C. The resultant product was electrophoresed on a 2.5% agarose gel and band intensity

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HinfI

HinfI from New England Biolabs

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New England Biolabs

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HinfI from New England Biolabs

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